Part:BBa_K2984029

L1c-PsaD-GLE-PETase-YFP-SP20-RBCS2

This vector is a part of the Chlamy-HUB-Collection. This part is composed of the PsaD promoter, the GLE secretion signal, the PETase enzyme, a YFP mVenus marker, the SP20 glycomodule for enhanced secretion and the Rbcs2 terminator. The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The YFP-mVenus tag can be used to detect the expression and secretion of the PETase.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1775
  Illegal PstI site found at 1989
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1314
  Illegal PstI site found at 1775
  Illegal PstI site found at 1989
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 4
  Illegal XhoI site found at 1038
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1775
  Illegal PstI site found at 1989
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1775
  Illegal PstI site found at 1989
  Illegal NgoMIV site found at 2466
  
• 1000
  COMPATIBLE WITH RFC[1000]

Characterization

Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii